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Abstract

VDAC Differential Interactome in Chicken Brain: Possible Hints to an Intrinsically Distinct Metabolism

The kinetic assembly of protein complexes containing the VDAC (voltage dependent anion channel) follows a quite different pattern in bovine brain in comparison with rat neuronal cells. In order to investigate if the differential expression of VDACs between chicken brain when compared with bovine and rat brain mitochondria was linked to a differential VDAC interactome, we utilized BN-PAGE of mitochondria treated with dodecyl maltoside, followed by a seconddimensional SDS-PAGE. Unusually perhaps, several VDAC interactant proteins in chicken brain were not members of VDAC interactome in rat and bovine brain, which possibly suggests that these cells exhibit intrinsically differential responses to events such as neuronal cell death, bioenergetics, oxygen consumption and oxidative insults.


Author(s):

Marcelo de Cerqueira Cesar



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